We'd like to understand how you use our websites in order to improve them. Register your interest. Peach Prunus persica is one of the most valuable fruit trees around the world including Iran. During spring and summer , severe branch dieback and canker was observed on peach trees in Hamadan province, Iran. The cankered peach twigs contained fruiting bodies of the fungus. A fungus was consistently isolated from infected tissues.
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We'd like to understand how you use our websites in order to improve them. Register your interest. Peach Prunus persica is one of the most valuable fruit trees around the world including Iran. During spring and summer , severe branch dieback and canker was observed on peach trees in Hamadan province, Iran.
The cankered peach twigs contained fruiting bodies of the fungus. A fungus was consistently isolated from infected tissues. It was already reported that C.
However, to our knowledge, this is the first report of C. Peach Prunus persica is an economically important deciduous tree belonging to the Rosaceae family producing 20 million tons of fruit per year worldwide. It is one of the most important fruit trees around the world including Iran. The peach orchards with dieback and canker symptoms were observed during spring and summer , in Hamadan province of Iran.
The symptoms included wood lesions and cankers with fruiting bodies from fungus Fig. In order to identify the causal agent of the disease, a total of 80 diseased twigs were collected from symptomatic trees. Small pieces, approximately 0.
Purification was done from three-day-old isolates by the mycelium tip culture technique and also in some cases, the single-spore technique was made using Adams et al. Cytospora chrysosperma.
The morphological characteristics were determined based on pure cultures grown on PDA. Conidiomata were examined on infected twigs, the structure and size of fruiting bodies, presence or absence of a conceptacle, and size and shape of spores also were recorded. Dilutions were performed with the spore masses obtained from fungus fruiting bodies, and drops of suspensions were placed on microscope slides. More than 30 fruiting bodies were sectioned and in total 50 spores derived from different fruiting bodies were selected randomly to measure their lengths and widths using a Leica light microscope.
These included colony characters and pigment production at 7, 14, and days after incubation. Colony color on the surface initially was white and then changed moderately to intense yellow or moderate orange yellow Fig. Teleomorph stage was not observed. Locules were multi-chambered with dark walls. Conidia hyaline, allantoid, aseptate, 3. The cultural and morphological characteristics of our isolates were in agreement with the description for C.
Raw sequences were edited in the BioEdit v. BLAST search analyses showed that the sequence data for two loci had the highest similarity to Cytospora chrysosperma. Clade stability was assessed using bootstrap replications Felsenstein Diaporthe eres was used as the outgroup in the phylogenetic analyses Arzanlou and Narmani Single Neighbor-Joining tree depicting the phylogenetic relationship between C.
Pathogenicity tests were performed on excised shoots in laboratory conditions as described by Arzanlou and Narmani In total, 15 healthy shoots a year-old were cut from trees. The wound was wrapped with Parafilm and for the control shoot, a plug of clean PDA was placed in the wound.
Inoculated shoots were placed in a plastic container containing moistened filter papers to keep the relative humidity high. The shoots were examined after three weeks to explore the progress of the disease. Longitudinal and cross sections were made from above and below the inoculation point. Wood necrosis was evident in the inoculated shoots Fig. No visible symptoms were observed on control shoots, whereas C. According to the results of the pathogenicity test, culture appearance, morphological characteristics and molecular analyses of the isolated fungus, the causal agent of the canker disease of P.
Disease caused by C. The potential of Cytospora group to cause yield losses in economically important crops warrants further study on these fungal pathogens. Since peach is an important tree and this pathogen leads to major damage, better understanding of this destructive disease would lead to the establishment of better disease control strategies.
It seems outbreak of canker by C. To our knowledge, this is the first report of canker disease caused by C. Australas Plant Pathol — Arzanlou M, Narmani A ITS sequence data and morphology differentiate Cytospora chrysosperma associated with trunk disease of grapevine in northern Iran. J Plant Prot Res — Appl Environ Microbiol — Nucleic Acids Symp Ser — Kimura M A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol — Mol Biol Evol — Plant Mol Biol Rep a—f.
Nucleic Acids Res — Academic Press, San Diego. Download references. Correspondence to Doustmorad Zafari. Reprints and Permissions. Bagherabadi, S. Morphological and molecular identification of Cytospora chrysosperma causing canker disease on Prunus persica. Australasian Plant Dis. Notes 12, 26 Download citation. Received : 22 November Accepted : 11 May Published : 24 May Search SpringerLink Search. Download PDF. Abstract Peach Prunus persica is one of the most valuable fruit trees around the world including Iran.
Full size image. You can also search for this author in PubMed Google Scholar. View author publications. Rights and permissions Reprints and Permissions. About this article. Cite this article Bagherabadi, S.
EPPO Global Database
A new disease, causing death of mature white poplar trees Populus alba L. The affected branches turned yellowish brown, and the inner bark turned black. The bark dried out and separated from the underlying wood. Later, copious, dark pycnidia developed on the dead bark. Under moist conditions, spore masses oozed out in long, reddish brown, coiled tendrils. The spores were hyaline, one-celled, and slightly curved, 1. A herbarium specimen was deposited at the U.
First Report of Cytospora Canker Caused by Cytospora Chrysosperma on White Poplar in Israel
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